Figure 5.

Localization of GFPs fused to N-terminal regions of SIG2 and SIG3. GFP fusion constructs with the N-terminal regions of SIG2 ORF (SIG2-GFP, C and G) and SIG3 ORF (SIG3-GFP, D and H), and the transit peptide of the small subunit of Rubisco (23) (RBCS-GFP, B and F), as well as GFP alone (23) (GFP, A and E), were introduced into tobacco leaves by particle bombardment. Guard cells were observed by using the MRC-1024 Confocal Imaging System (480×) with excitation at 488 nm and emission at 520 nm (A–D), as well as excitation at 647 nm and emission at 666 nm (E–H). The same objects are shown in each pair of upper and lower panels.