Representative plants of wild-type (WT),
sos1, and sos3 mutants that were exposed
to high-salt (100 mM NaCl) or low-K+ (20 μM
K+) stresses. Seedlings were grown on vertical MS agar
plates for 4 days (root length ≅ 1.5 cm) and then transferred to
vertical agar plates containing control (A, MS
nutrients), 100 mM NaCl (B, MS + 100 mM NaCl), or
modified MS (see Materials and Methods) with 20 μM
K+ and 0.15 mM (C) or 3 mM Ca2+
(D) for 7 days. The growth of both sos1 and
sos3 was inhibited by either NaCl (B) or low
K+ (C). However, the growth of
sos3 on low K+ was restored by increased
Ca2+ (D). (Bar = 1
cm.)