Figure 6.

sos1sos3 double mutant resembles sos1 both in salt sensitivity and in response to Ca²⁺. (A) Growth of wild-type (□), sos1 (▪), sos3 (░⃞), and sos1sos3 (▥) seedlings on MS medium, MS medium supplemented with extra 10 or 25 mM NaCl. (B) Growth of wild-type (□), sos1 (▪), sos3 (▥), and sos1sos3 (▥) seedlings on MS (20 mM K⁺, 3 mM Ca²⁺), low K⁺–low Ca²⁺ (20 μM K⁺, 0.15 mM Ca²⁺), or low K⁺–high Ca²⁺ (20 μM K⁺, 3 mM Ca²⁺) media. Growth measurement was conducted on the seventh day after the transfer. For Ca²⁺-responsive treatment (B), the measurement was taken 3 days after seedling transfer to minimize the effect of residual K⁺ and Ca²⁺ carried by the plants from MS medium. [Bars = SD (n = 15).]