Figure 1.

The effects of Rho and Rac on cadherin receptors is influenced by the maturation of the intercellular contacts. Keratinocytes grown in low calcium medium were transferred to standard medium to induce calcium-dependent cell–cell contacts for 3 h (newly formed junctions) (A and B) or alternatively, cells were grown in standard medium (mature junctions) (C and D). After microinjection of a dominant negative form of Rac (N17Rac) to block endogenous Rac, cells were incubated for further 30 min. E-cadherin was visualized by staining with HECD-1 followed by FITC-conjugated anti-mouse (B and D); injected cells are seen in panels A and C. Bar, 50 μm.