Figure 6.

N17Rac is unable to disrupt cadherin-mediated adhesion in fibroblasts. L-cells expressing full-length E-cadherin were microinjected with C3 (A and B) or a dominant negative form of Rac (N17Rac; C and D), incubated for 1 or 2 h (A–D and our unpublished observations) and stained for E-cadherin (B and D). Quiescent Swiss 3T3 fibroblasts were also injected with N17Rac and incubated for up to 2 h (E and F and our unpublished observations) and stained with an anti–pan-cadherin antibody (F). Similar results were obtained after 1 or 2 h of incubation with either cell type. Injected cells are shown in panels A, C, and E. Arrows point to the absence (B) or presence (D and F) of cadherin at sites of cell–cell contacts. Bar, 50 μm.