Figure 3. WT and K⁴⁰⁹A rHsp65 treatment in anti-Hsp65 antibody response.

Anti-Hsp65 IgG1 [A] and IgG2a [B] antibody production, respectively, in the serum of treated and untreated [NZBxNZW]F₁ mice of 50 and 80 days-old [n = 5–6/group]. IgG2a/IgG1 anti-Hsp65 antibody ratio [C] in WT, K⁴⁰⁹A and untreated groups in 80 days-old F₁ mice. Antibodies were measured by isotype-specific ELISA in serum of 50 and 80 days-old mice. Untreated group has been the reference group for unpaired t-test analysis; *p<0.05. Results are representative of 3 independent experiments.