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S427 Trypanosoma brucei brucei trypanosomes were incubated in vitro with 500 nM of DB351 for various time points between 1 hour and 24 hours. Trypanosomes were washed and resuspended in freshly isolated mouse blood. Dry smears were prepared and examined using a Nikon Microphot microscope with a UV2A cube. Nuclei are found in the center of trypanosomes, and the kinetoplast is found at the end of trypanosomes.
