Skip to main content
. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Biochimie. 2008 Mar 4;90(7):999–1014. doi: 10.1016/j.biochi.2008.02.017

Figure 6.

Figure 6

(A) Sequence of trans and cis oligomers used in the gel electrophoresis experiments. (B) Native PAGE of trans (lanes 2 and 4) and cis ligation ladders (lanes 1 and 3) and 20-bp marker (M) in the presence of DB351. Circular products (C) and linear ligation products (L) are marked, where L2 corresponds to the 42-bp dimer, L3 to the 63-bp, and so forth. (C) Plot of relative mobility (where RL= apparent length/actual length) versus actual length (Lact) for trans (red circle) and cis (red triangle) ligation ladders without DB351 and trans (blue circle) and cis (blue triangle) with DB351. Only linear products were used to calculate RL, and missing points correspond to linear ligation products indistinguishable from circular products.