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DAF−/−/Crry−/−/C3−/− platelets are more sensitive to alternative pathway complement attack than DAF−/−/Crry−/−/C3−/− erythrocytes. FACS analysis of C3 deposition on DAF−/−/Crry−/−/C3−/− platelets and erythrocytes after 20 minutes (A-C) or 60 minutes (D-F) of incubation with various concentrations of wild-type mouse plasma in 10 mM EGTA-MgCl2. MFI indicates mean fluorescence intensity. Bars in panels C and F represent means (± SEM) of triplicate assays.
