Figure 4.

Pulse–chase analysis of gp180 and gp180 deletion mutants. Stable transfectants expressing similar levels of gp180 or gp180 deletion constructs (depicted in Figure 1) were metabolically labeled for 15 min with [³⁵S]Met. Cells on replicate plates were collected either immediately after the pulse (0′) or after the chase times indicated in minutes (60′, 90′, and 120′). Proteins were isolated from detergent extracts using the anti-gp180 antiserum and resolved by SDS-PAGE under reducing conditions on 7% polyacrylamide gels. This analysis was performed with two single clones for each construct, with comparable results.