Figure 5.

Top, steady-state localization of the two point mutations of gp180 (ADA and AHRL). Stable transfectants were fixed, permeabilized, and costained for gp180 and syntaxin 6, as described in Figure 3 legend. Bottom, antiserum uptake for cells expressing full-length gp180, the Δ24 deletion mutant, and the ADA and AHRL point mutants. Cells were incubated in media containing gp180 antiserum (1:500) at 37°C for 1 h. Then, cells were fixed and permeabilized, and antiserum to gp180 was detected with Texas Red-conjugated goat anti-rabbit IgGs. Steady-state levels of syntaxin 6 (i.e., not internalized antibodies) were detected as described in Figure 3 legend. Bar, 10 μm.