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. 2008 Jul 4;9(8):810–818. doi: 10.1038/embor.2008.121

Figure 5.

Figure 5

Interaction between Yku and cyclin-dependent kinase in the regulation of double-strand break resection. (A–C) YEP+raf nocodazole-arrested cell cultures of wild-type JKM139 and yku80Δ, GAL-SIC1ΔNT and GAL-SIC1ΔNT yku80Δ derivative strains were transferred to nocodazole-containing YEP+raf+gal (time zero). (A) Analysis of DSB resection as described in Fig 1B. (B) Densitometric analysis of the representative experiment shown in (A). Three independent experiments were performed with similar results. (C) Western blot analysis of protein extracts with Rad53 antibodies. (D,E) YEP+raf α-factor-arrested cell cultures of wild-type JKM139 and GAL-CLB2 or yku80Δ derivative strains were transferred to α-factor-containing YEP+raf+gal (time zero). (D) Analysis of DSB resection as described in Fig 1B. (E) Densitometric analysis of the representative experiment shown in (D). Three independent experiments were performed with similar results. (F,G) ChIP analysis of Yku70 binding to an HO-induced DSB. (F) YEP+raf α-factor- (G1) or nocodazole-arrested (G2) cell cultures of a JKM139 derivative strain expressing the YKU70-MYC allele were transferred to α-factor- or nocodazole-containing YEP+raf+gal, respectively (time zero). (G) YEP+raf α-factor-arrested cell cultures of wild-type and GAL-CLB2 isogenic strains expressing the YKU70-MYC allele were transferred to α-factor-containing YEP+raf+gal (time zero). In both (F) and (G), densitometric data from three independent experiments were expressed as in Fig 2F. Error bars indicate s.d. ChIP, chromatin immunoprecipitation; DSB, double-strand break; Exp, exponentially growing cells; wt, wild type; YEP, yeast extract peptone.