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. 2008 Jun 20;9(8):781–787. doi: 10.1038/embor.2008.112

Figure 4.

Figure 4

Loss of asymmetrical Ash1 distribution in She3N–S2 cells. (A) Cellular distribution of nuclear Myc-tagged Ash1 was categorized as asymmetrical, partly asymmetrical or symmetrical. (B) Ash1-Myc9 distribution in a wild-type strain (She2; black columns), an She3N–S2 strain (She3N–She2; grey columns) and a strain expressing an She3–She2 fusion protein containing a tandem nuclear localization signal at the N terminus (2 × NLS-She3N–She2; white columns) was quantified by counting 351 binucleate cells. DAPI, 4,6-diamidino-2-phenylindole; NLS, nuclear localization signal.