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. 2008 Aug 12;105(33):12081–12086. doi: 10.1073/pnas.0804175105

Fig. 1.

Fig. 1.

Biochemical assessment of the Fe response status and Fe accumulation of Arabidopsis (Col-0): immunoblot showing IRT1 protein accumulation in roots (A), ferric chelate reducatse activity in roots (B), and Fe accumulation in leaves (C) of Arabidopsis Col-0 plants grown under Fe-deficient (1 μM FeHBED) and Fe-sufficient (10 μM FeHBED) conditions. Data for the ferric chelate reductase activity represents the mean ± SD of three batches of roots from approximately three plants each. The immunoblot represents IRT1 protein in a pooled sample of roots from ≈18 plants with 10 μg of total protein loaded in each lane, and the experiment replicated twice with a representative blot shown. Box plots represent the interquartile range (IQR), the bisecting line represents the median, the whiskers represent 1.5 times the IQR, the dots represent outlier points, and the data are from 21 individual plants. All plants were grown for 5 weeks in soil.