Table 1.
Prediction of Fe status of Arabidopsis Col-0 plants grown in altered nutrient environments
| Element (Hoagland's) | Level | Concentration | Plants predicted to be Fe-deficient, % |
|---|---|---|---|
| P (250 μM) | Low | 62.5 μM | 7* |
| High | nd | nd | |
| Mg (0.5 mM) | Low | 0.0 | |
| High | 4 mM | 0 | |
| Mn (2.25 μM) | Low | 0.0 | 0 |
| High | 18.3 μM | 11* | |
| B (11.5 μM) | Low | 0.0 | 0 |
| High | 90 μM | 11* | |
| Ca (1 mM) | Low | 0.0 | 0 |
| High | 8 mM | 0 | |
| NO3 (2.5 mM) | Low | 0.0 | 0 |
| High | 24 mM | 0 | |
| Zn (0.2 μM) | Low | 0.0 | 0 |
| High | 1.6 μM | 11* | |
| Cu (75 nM) | Low | 0.0 | 0 |
| High | 0.6 μM | 0 |
Each element in the nutrient solution was either increased or decreased/removed from the standard concentration in our normal Hoagland's nutrient solution (shown below element name). The Fe model was used to predict the Fe response status of the plants. All of the Arabidopsis (Col-0) plants grown at the same time with the normal nutrient solution had <25% of the plants predicted to be in the low-Fe state. nd, not determined.
*Values do not exceed the miscall rate predicted from the modeling and are therefore not significant. Nine plants were grown in each treatment except for P where n = 108.