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. Author manuscript; available in PMC: 2008 Aug 12.
Published in final edited form as: Biochemistry. 2006 Jul 4;45(26):8163–8173. doi: 10.1021/bi060446d

FIGURE 4.

FIGURE 4

The pH dependence of enzyme activity. All reactions (50 μL total volume) were carried out in 50 mM potassium phosphate buffer for pH 6.0–7.6, in 20 mM Tris·HCl buffer for pH 8.0–8.8, and in 50 mM glycine-NaOH solution for pH 9.6–10.0. (A) Reaction included UDP-GlcNAc (2), NAD+, and TviB. (B) Reaction included UDP-GlcNAcA (4) and TviC. See the Experimental Procedures for details.