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. Author manuscript; available in PMC: 2008 Aug 12.
Published in final edited form as: Biochemistry. 2006 Jul 4;45(26):8163–8173. doi: 10.1021/bi060446d

FIGURE 8.

FIGURE 8

Kinetics analyses of the epimerization of UDP-GlcNAcA and UDP-GalNAcA by TviC using FPLC. The initial rates of epimerization were determined by measuring the slope of the plot of amount of product formation versus time. The data were fitted to Michaelis-Menten equation by nonlinear regression using Grafit 5. (A) Reactions were performed with UDP-GlcNAcA (4) as the substrate using 27 pmol of TviC. (B) Reactions were performed with UDP-GalNAcA (5) as substrate using 53 pmol of TviC. All reactions were conducted at room temperature in 40 μL of 50 mM potassium phosphate buffer containing 2 mM MgCl2, pH 8.0. See Experimental Procedures for details.