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. Author manuscript; available in PMC: 2008 Aug 12.
Published in final edited form as: Biochemistry. 2006 Jul 4;45(26):8163–8173. doi: 10.1021/bi060446d

Table 1.

Kinetic Parameters for Reaction Catalyzed by TviB Determined by the Spectrophotometric Assaya

Substrate Km
(mM)		 Vmax
(mM/min)		 TviB
mM		 kcat
(min−1)		 kcat/Km
(mM−1min−1)
UDP-GlcNAc (2) 0.077±0.009 0.031±0.002 2.0×10−3 15.5±1.0 198.8±19.1
NAD+ 0.276±0.052 0.031±0.002 2.0×10−3 15.5±1.0 56.2±8.0
a

See Experimental Procedures for details