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. Author manuscript; available in PMC: 2008 Aug 12.
Published in final edited form as: Biochemistry. 2006 Jul 4;45(26):8163–8173. doi: 10.1021/bi060446d

Table 2.

Kinetic Parameters for Reactions Catalyzed by TviC Determined by the HPLC Assay and Capillary Electrophoresis Assaya

Substrate Km
(mM)		 Vmax
(mM/min)		 TviC
mM		 kcat
(min−1)		 kcat/Km
(mM−1min−1)
UDP-GlcNAcA (4)b 0.020±0.001 0.0015±0.00001 2.7×10−5 56.8±0.5 2871.6±110.8
UDP-GalNAcA (5)b 0.042±0.002 0.0021±0.00003 5.3×10−5 39.1±0.6 931.3±43.3
UDP-GlcNAc (2)c 0.051±0.003 0.0038±0.00005 2.7×10−4 14.2±0.2 281.6±12.6
UDP-GalNAc (3)c 0.071±0.005 0.0068±0.0001 1.3×10−4 52.6±0.9 741.7±38
a

See Experimental Procedures for details.

b

Values listed were determined by HPLC assays.

c

Values listed were determined by the capillary electrophoresis assays.