Skip to main content
. Author manuscript; available in PMC: 2008 Aug 12.
Published in final edited form as: Biochemistry. 2007 Jun 22;46(28):8401–8413. doi: 10.1021/bi700286a

FIGURE 2.

FIGURE 2

Anaerobic photoreduction of IDI-2. (A) The as-purified IDI-2 (396 μM) in 0.1 M potassium phosphate buffer (pH 7.0) containing 20 mM EDTA and 1.2 μM 5-deazariboflavin was irradiated with a 300-watt slide projector lamp (see Experimental Procedures) at 25 °C under anaerobic conditions. Spectra (top to bottom) were recorded after 0, 9, 13, 24, 57, 167, and 1382 sec of irradiation, respectively. Inset figure shows a plot of absorbance at 583 nm versus absorbance at 452 nm during reduction. The solid lines represent extrapolation of absorbance values to 100% neutral semiquinone. (B) UV-vis absorption spectra for oxidized (solid line) and reduced (dashed line) IDI-2 obtained from the photoreduction data in Figure 2A.