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Dissociation Constants Obtained from Ultrafiltration and UV-Vis Difference Spectroscopy Experiments
| Kd (μM) | ||
|---|---|---|
| Flavin Cofactor | Ultrafiltration | UV-vis Difference Spectroscopy |
| FMN | 34 ± 3 | 24 ±5 (λ = 440nm) 18 ±5 (λ = 493nm) |
| 1-deazaFMN | N.D.a | 97 ± 19 (λ = 333 nm) |
| 5-deazaFMN | N.D.a | 16 ± 3 (λ = 430nm) |
Not determined.
