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. Author manuscript; available in PMC: 2008 Aug 12.
Published in final edited form as: Biochemistry. 2007 Jun 22;46(28):8401–8413. doi: 10.1021/bi700286a

Table 2.

Steady-State Kinetic Parameters for IDI-2 from Staphylococcus aureus

Conditions kcat (sec−4) Km for IPP (μM) kcat/Km (M−1 sec−1)
Aerobic with 5 mM NADPH as reducing agent 0.065 ± 0.008 4.8 ± 10.7 1.4 × 104
Aerobic with 10 mM dithionite as reducing agent 0.57 ± 0.02 11.9± 1.8 4.8 × 104
Anaerobic with 5 mM NADPH as reducing agent 0.69 ± 0.03 16.8 ±3.0 4.1 × 104