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SDS-PAGE showing pure LLO elutions (500 ml bacterial culture) using the double sonication approach. Pure LLO was eluted from the Ni-NTA affinity gel after 4 h-0.2% nisin induction at 30°C and double sonication treatment of the induced cells as described in the Methods section. Lanes 1–3: elutions after first sonication treatment. Lanes 4–8: elutions after second sonication treatment.
