Figure 2.

Rab25 and Rab11a colocalize in Rab25-transfected cells. MDCK cells stably expressing Rab25 were cultured on permeable supports. Fixed cells were triple-stained with antibodies against Rab11a, Rab25, and ZO-1 with detection by secondary antibodies conjugated with Cy5, Cy2, and Cy3, respectively. Maximum intensity projections were constructed from 40 confocal optical sections (0.3 μm each). Rab25 staining overlapped with Rab11a staining in most cells (arrows). In many cells, instead of a single nidus of immunoreactivity for Rab11a/Rab25, several brightly staining foci were observed in the apical region of the cell. Bar, 4 μm.