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. 1999 Jan;10(1):47–61. doi: 10.1091/mbc.10.1.47

Figure 6.

Figure 6

GTPase activity in Rab11 family members. The GTPase activities of (A) recombinant Rab25 and (B) recombinant Rab11a and Rab11aQ70L were assessed in vitro. (A) GTPase activity was assessed as described in MATERIALS AND METHODS in 1 μg of recombinant GST-Rab25 in the absence (□) or presence (▪) of gastric cytosol as a GAP donor. (B) GTPase activity was assessed in recombinant His-tagged Rab11a (circles) and His-tagged Rab11aQ70L (squares) in the absence (open symbols) or presence (closed symbols) of gastric cytosol as a GAP donor. All results represent the mean + SEM of three separate experiments each with duplicate samples. The results indicate that Rab25, Rab11a, and Rab11aQ70L are all active GTPases.