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. 2008 Jun 11;17(17):2723–2737. doi: 10.1093/hmg/ddn174

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Published by Oxford University Press 2008

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Degradation of autophagosomes is delayed in MLIV fibroblasts. (A) Control and MLIV fibroblasts were starved in EBSS media for 3 h and then allowed to recover for 20 or 40 min in complete medium. Cells were then fixed and LC3 localization was examined by immunofluorescence. Scale bar = 10 µm. (B) Percentage of cells showing more than 20, between 10 and 20 or less than 10 LC3-positive structures after a 20 min recovery (left panel) or 40 min of recovery (right panel).