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. 2008 Aug 14;118(9):3038–3050. doi: 10.1172/JCI33337

Figure 5. α/β-null L-CFCs show altered drug sensitivity.

Figure 5

(A) L-CFCs were treated with the indicated inhibitors for 24 hours and assessed for apoptosis (subdiploid DNA, left panel) and cycling cells (% S/G2/M phase, right panel) by flow cytometry. Differences in treatment effects between β-null and α/β-null were analyzed by 2-way ANOVA (*P < 0.05, **P < 0.01) and 1-way ANOVA for wortmannin effects compared with untreated controls (§P < 0.001). Mean values ± SEM are shown; n = 4, using 4 different clones. (B) L-CFCs (blue, β-null; red, α/β-null) were treated with or without 1 μM or 2 μM imatinib for 16 hours or 24 hours and analyzed for apoptosis. Cells that stained positive for annexin V and negative or positive for 7-AAD are reported as mean percentage ± SEM; n = 3 independent experiments.