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. 2008 Aug 14;118(9):3038–3050. doi: 10.1172/JCI33337

Figure 6. α/β-null L-CFCs maintain mTOR signaling via class IA PI3K–independent mechanisms.

Figure 6

(A) p190 L-CFCs were treated for 2 hours with the indicated inhibitors, and the percentage of p-rS6 was quantified by ImageJ (upper p-rS6 band was normalized to β-actin; representative of 4 independent experiments and multiple clones). (B and C) L-CFCs were treated with the indicated inhibitors and/or leucine starved for 2 hours and immunoblotted with the indicated antibodies. n = 3, using 3 clones. 3-MA, 3-methyladenine. (D) Cells of the indicated genotypes were starved in leucine-deficient medium (containing 10% dialyzed FCS) for the indicated times or treated with rapamycin (1.5-hour treatment). Control samples were incubated with leucine-sufficient medium (with matched dialyzed serum). n = 3, using 2 clones.