Skip to main content
. 2008 Aug 14;118(9):3087–3097. doi: 10.1172/JCI35781

Figure 4. Hsp104 reduces the number of phosphorylated α-syn inclusions.

Figure 4

(A–C) Robust expression of human α-syn was detected with both RG syn and (D–I) the human specific LB509 Abs. (G–I) Higher magnification revealed the accumulation and aggregation of human α-syn in both the cell bodies (arrows) and neurites (arrowheads). (H and I) Note that there are fewer dense puncta in neurites in the A30P/Hsp104 condition. (J) Sections of the substantia nigra from the noninjected side and (K) from rats expressing A30P/YFP or (L) A30P/Hsp104 were immunostained with Ab specific for phosphorylated Ser 129 of α-syn. The substantia nigra of animals overexpressing either A30P/YFP or A30P/Hsp104 reveals the presence of hyperphosphorylated inclusions reminiscent of Lewy bodies. Scale bar: 300 μm (A–F); 40 μm (G–I); 50 μm (J–L). (M) Quantitation of neurons containing Pser129-positive aggregates in the substantia nigra of rats expressing A30P/YFP or A30P/Hsp104. Values represent mean ± SEM; n = 6 animals per group; P < 0.05.