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. 2008 Aug 14;118(9):3087–3097. doi: 10.1172/JCI35781

Figure 6. Hsp104 remodels α-syn A30P preamyloid oligomers.

Figure 6

(A–D) Purified α-syn A30P preamyloid oligomers (0.5 μM) were incubated without or with Hsp104 or Hsp104K218T:K620T (10 μM) plus ATP and regeneration system for 1 hour at 37°C. Reactions were spotted on nitrocellulose and probed with anti-oligomer Ab or anti–α-syn Ab (A). (B) Alternatively, reactions were processed for EM (scale bar: 25 nm) or (C) depleted of Hsp104 and passed through a 100-kDa molecular weight filter. (C and D) Retentate and filtrate fractions were collected and analyzed by SDS-PAGE, and the amount of A30P in the filtrate was determined by densitometry. Values represent mean ± SD; n = 4 (D).