Skip to main content
. 1999 Jan;10(1):63–75. doi: 10.1091/mbc.10.1.63

Figure 5.

Figure 5

Secretion of highly glycosylated α-factor precursor in ypt6/sys3 double deletion mutants. MATα strains carrying gene deletions as indicated were grown to exponential phase and labeled for 30 min at 25°C with Tran35S-label. Culture media were collected, and α-factor was immunoprecipitated with anti-α-factor antiserum and analyzed by SDS-PAGE on 15% gels. α (p), highly glycosylated α-factor precursor; α (m), mature α-factor.