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Secretion of highly glycosylated α-factor precursor in ypt6/sys3 double deletion mutants. MATα strains carrying gene deletions as indicated were grown to exponential phase and labeled for 30 min at 25°C with Tran35S-label. Culture media were collected, and α-factor was immunoprecipitated with anti-α-factor antiserum and analyzed by SDS-PAGE on 15% gels. α (p), highly glycosylated α-factor precursor; α (m), mature α-factor.
