Table 1.
Bacterial strains and plasmids.
| Strains or plasmids | Genotype | Reference/source |
|---|---|---|
| E. coli | ||
| β2155 | ThrB1004 pro thi strA hsdS lacZΔM15 (F' lacZΔM15 lacIq trajD36 proA+ proB+) ΔdapA::erm pir.:RP4, Ermr, Kmr | Dehio and Meyer (1997) |
| SM10λpir | Thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpirR6K; Kmr | Miller and Mekalanos (1988) |
| SS6005 | F− thi leu rpsL Δ(argF-lac)U169 Φ(udp-lac)8 (λRS45) | Barbier and Short (1992) |
| SS6018 | SS6005, cytR::Tn10dTet | Barbier and Short (1992) |
| V. cholerae | ||
| MO10 | 1992 clinical isolate of V. cholerae O139 from India, Smr | Waldor and Mekalanos (1994) |
| PW324 | MO10ΔcytR, Smr | This study |
| PW328 | MO10Δvps, Smr | This study |
| PW329 | MO10ΔcytRΔvps, Smr | This study |
| PW357 | MO10lacZ::vpsLp→lacZ, Smr | This study |
| PW358 | PW324lacZ::vpsLp→lacZ, Smr | This study |
| PW386 | PW324lacZ::udp1p→lacZ, Smr | This study |
| PW387 | MO10lacZ::udp1p→lacZ, Smr | This study |
| Plasmids | ||
| pBSL180 | OriR6K mobRP4 lacI pTac tnp mini-Tn10Km; Kmr, Apr | Alexeyev and Shokolenko (1995) |
| pWM91 | OriR6K OriT sacB lacZα, Apr | Metcalf et al. (1996) |
| pAJH1 | pWM91 carrying a fragment of cytR harbouring an internal, unmarked deletion | This study |
| pAJH5 | pWM91 carrying a fragment of a vps operon harbouring an internal, unmarked deletion | This study |
| pAJH3 | A pBR322 derivative containing the promoter and coding sequence of cytR; Tcr Apr | This study |
| p6891MCS | pBR327 with 8 kbp Sau3A fragment of V. cholerae lacZ gene interrupted by a multiple cloning site | Butterton et al. (1995) |
| PAJH8 | p6891MCS derivative with udp1p→lacZ inserted in the multiple cloning site | This study |
| pAJH2 | p6891MCS derivative with vpsLp→lacZ inserted in the multiple cloning site | This study |
| pSMC2 | pKEN carrying a bright mutant of gfp and 1.8 kb stabilizing fragment from pUC181.8; Apr | Bloemberg et al. (1997) |