TABLE 1.
Scp1 mutations identified in selection
| Scp1 Residue | Mutations (# Times)b | Scap Mutation Tested | SREBPc Activation |
|---|---|---|---|
| F245 | R(11), A(1), E(1), S(1) | F296R | − |
| Y247 | P(5), C(1), G(1), H(1), R(1) | Y298P | WT |
| Y249 | N(3), P(3), R(3), I(1), l(1), S(1), V(1) | Y300N | WT |
| R259a | H(4), C(2) | K310H | WT |
| A260a | V(4), T(4), D(1) | S311V | WT |
| K261 | I(4), H(2), Q(2), T(2), V(2), C(1), S(1) | K312I | WT |
| G263 | l(4), P(3), A(2), R(2), D(1), E(1), I(1) | G314L | WT |
| L264 | P(10), R(4) | L315P | WT |
| F286 | l(9), I(3) | F337L | WT |
| V326 | R(7), Y(1) | V377R | WT |
| F369 | R(12), G(1), V(1) | F420R | WT |
| L380 | R(16) | L431R | − |
| F384 | V(2), C(1), G(1), I(1), l(1), S(1), T(1) | F435V | WT |
| F385 | R(9), P(6) | F436R | − |
| V388 | G(4), S(3), l(2), D(1), E(1) | V439G | + |
| L389 | P(6), Δ389(1) | L440P | − |
| S390 | l(4), K(2), Y(2), R(1), W(1) | S441L | WT |
| D392 | E(9), H(2), N(1), R(1) | D443E | WT |
| I393 | P(8) | I444P | WT |
| R394 | P(6), D(4), E(1), N(1), S(1), T(1) | R445P | WT |
| R395 | l(2), A(2), G(2), I(2), C(1), E(1), N(1), S(1) | R446L | WT |
| E397 | P(10) | E448P | − |
| L398 | W(5), P(3), R(1) | L449W | − |
a
Residues identified as second-site mutations in original selection.
b
Mutations in bold were remade and confirmed as Sre1-activating mutations.
c
Class abbreviations for SREBP-2 activation: none (−), wild-type (WT), constitutive (+).