Fig. 7.

FoxO1 mediates insulin inhibition of Cyp7a1 in rat livers. A: Real-time PCR assays of Cyp7a1 mRNA expression in the livers of rats of control, streptozotocin (STZ), or STZ plus insulin group. Results are expressed as mean ± SEM. Student's t-test was used to compare two groups. A P value < 0.05 was considered to be significant. * Statistically significant difference (P < 0.05, n = 5), STZ-treated vs. vehicle control; ** statistically significant difference (P < 0.05, n = 5), STZ + insulin vs. STZ. B: Rat liver nuclei were used in chromatin immunoprecipitation (ChIP) assay. ChIP assays were performed as described in Materials and Methods. Ten percent lysate was used as “input”. Nonimmuno IgG was used as background control. PCR amplified DNA fragments were analyzed on agarose gel.