Table 3.
Amino acid concentrations in μmol/g FW ± standard error in shoot tips after constant temperature preculture (CT, 22°C constant temperature, 7 days) and alternating temperature preculture (AT, 22/8°C day/night temperature, 7 days)
| CT | AT | |||||||
|---|---|---|---|---|---|---|---|---|
| S. tuberosum ‘Désirée’ | S. tuberosum ‘King Edward’ | S. acaule | S. demissum | S. tuberosum ‘Désirée’ | S. tuberosum ‘King Edward’ | S. acaule | S. demissum | |
| Asparagine | 1.28 ± 0.18 | 12.99 ± 1.75 | 3.31 ± 0.19 | 22.96 ± 2.57 | 8.03 ± 0.53* | 13.76 ± 1.66 | 5.49 ± 0.55* | 21.84 ± 2.59* |
| Aspartate | 0.39 ± 0.02 | 1.63 ± 0.18 | 0.58 ± 0.03 | 3.21 ± 0.30 | 0.33 ± 0.02* | 1.68 ± 0.11 | 0.79 ± 0.07* | 2.88 ± 0.16 |
| Glutamate | 0.69 ± 0.04 | 2.88 ± 0.27 | 0.90 ± 0.07 | 4.99 ± 0.29 | 0.52 ± 0.04* | 2.84 ± 0.17 | 0.99 ± 0.08 | 3.92 ± 0.29* |
| Glutamine | 0.80 ± 0.09 | 4.78 ± 0.52 | 4.22 ± 0.26 | 8.66 ± 0.56 | 1.50 ± 0.11* | 4.52 ± 0.26 | 2.79 ± 0.27* | 6.40 ± 0.28 |
| Glycine | 0.20 ± 0.02 | 0.35 ± 0.03 | 0.78 ± 0.06 | 0.69 ± 0.07 | 0.13 ± 0.02* | 0.42 ± 0.04 | 0.33 ± 0.05* | 0.40 ± 0.03 |
| Proline | 2.30 ± 0.23 | 4.91 ± 0.47 | 8.71 ± 0.88 | 8.78 ± 1.70 | 0.27 ± 0.03* | 5.17 ± 1.11 | 2.62 ± 0.25* | 6.84 ± 0.77 |
| Serine | 0.34 ± 0.02 | 2.53 ± 0.24 | 1.18 ± 0.07 | 4.69 ± 0.35 | 0.24 ± 0.02* | 3.00 ± 0.23 | 0.68 ± 0.05* | 4.04 ± 0.29* |
Results are shown as means of three experiments with 7–10 repetitions each, one repetition consisting of 20–50 shoot tips. Values in bold with asterisk mark significant changes after AT (P < 0.02)