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. Author manuscript; available in PMC: 2008 Aug 13.
Published in final edited form as: Nat Neurosci. 2008 Apr 6;11(5):538–540. doi: 10.1038/nn.2104

Figure 1.

Figure 1

preBötC neurons infected by AAV2 containing a Syn or Sst promoter. (a) EGFP expression (green) in adult rat preBötC (yellow circle) 3 weeks after microinjection of Syn-EGFP AAV2. 7N, facial nucleus; AmbC, ambiguous nucleus compact; BötC, Bötzinger Complex; LRt, Lateral reticular nucleus. (b) Syn-AlstR-EGFP AAV2–infected preBötC cells (green) were NeuN immunoreactive (red); that is, they were neurons. (c) Some Syn-AlstR-EGFP AAV2–infected preBötC neurons (green) were NK1R immunoreactive (red). (d) Sst-EGFP AAV2–infected preBötC neurons (green) were Sst immunoreactive (red). (e) Small portion of Sst-AlstR-EGFP AAV2–infected preBötC neurons (green) were NK1R immunoreactive (red). Images were obtained using a confocal microscope. Insets are high magnification of indicated neurons. Venn diagrams represent the relative number of neurons and the overlap of the relevant markers (b–e) normalized to NeuN immunoreactivity (n ≈ 3,000 neurons on each side of the preBötC). Yellow arrows represent neurons coexpressing and white arrows represent neurons not coexpressing the relevant markers. Scale bars represent 50 µm.