. Author manuscript; available in PMC: 2008 Aug 13.

Published in final edited form as: Biochemistry. 2005 Sep 27;44(38):12896–12904. doi: 10.1021/bi050762c

Table 1.

New mutational changes made in Tet(L) and Tet(K)^a

Protein	Mutant	Primer Sequence 5'-3'
Tet(L)	E152D	`CTGGTAGCAATGGGAGATGGTGTTGGGCCAGCT`
	G155A	`ATGGGAGAAGGTGTTGGCCCAGCTATTGGCGGA`
	G155C	`ATGGGAGAAGGTGTTTGCCCAGCTATTGGCGGA`
	G302A	`GGCATTATTTTCCCCGCAACAATGAGTGTCATCA`
	G302C	`GGCATTATTTTCCCCTGTACAATGAGTGTCATCA`
	G398C	`AGCTTTTTATCAGAGTGTACGGGTATTGCGA`
	G400C	`TTATCAGAGGGAACGTGTATTGCGATTGTAGGC`
	G405C	`GGTATTGCGATTGTATGCGGTTTATTATCTATCGG`
	G406C	`ATTGCGATTGTAGGCTGTTTATTATCTATCGGCTTC`
	P121C	`GCAGCCGCATTCTGCGCTCTTGTGATGGTT`
	P156A	`GGAGAAGGTGTTGGGGCAGCTATTGGCGGAATG`
	P156C	`GGAGAAGGTGTTGGGTGTGCTATTGGCGGAATG`
	P156G	`GGAGAAGGTGTTGGGCCAGCTATTGGCGGAATG`
	P175A	`TATTTGCTGCTTATTGCAACTGCAACAATTATC`
	P175G	`TATTTGCTGCTTATTGGAACTGCAACAATTATC`
	P183C	`TATTTGCTGCTTATTTGCACTGCAACAATTATC`
	P301S	`AGCGGCATTATTTCCTCCGGAACAATGAGTGTC`
	R110K	`ATTCTCATTCTAGCCAAATTTATTCAAGGAATTGG`
	T303A	`CATTATTTTCCCCGGAGCAATGAGTGTCATCATCTT`

Tet(K)	G155C	`CTTTAGGTGAAGGGTTATGTCCTTCAATAGGGGGA`
	G155S	`CTTTAGGTGAAGGGTTAAGTCCTTCAATAGGGGGA`
	P156C	`GGTGAAGGGTTAGGTTGTTCAATAGGGGGAATA`

The bold letters in the primer sequence indicate the mutation introduced.