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Methylation of loci of the ERα promoter changes following MCAO in vehicle-treated (A) and estradiol-treated female (B), but not male (C) rats 24 hours after MCAO. Genomic DNA was extracted from vehicle or estradiol-treated OVX female and male rats and methylation-specific PCR was conducted using primers specific for several CpG islands within the rat ER-α promoter. These studies were repeated 3 times.
