Figure 2.

Cells of a wild-type strain (W303–1A) transformed with either YEpPDE1 (PM851), YEpPDE2 (PM852), or the empty vector YEplac195 (PM850) were grown in SD glucose-uracil medium until OD₆₀₀ = 2.0–2.5, after which the OD was adjusted to 2 with the same medium. The cell suspension was heat shocked for 30 min at 50°C in a water bath. Treated and untreated cell suspensions were spotted on YPD plates and incubated at 30°C for 2 d. Serial dilutions were made with a factor of 10.