Table 1.
Saccharomyces cerevisiae strains used in this work
| Strain | Genetic background | Relevant genotype | Complete genotype | Source and/or reference |
|---|---|---|---|---|
| W303-1A | W303-1A | wild type | MATa leu2-3, 112 ura3-1 trp1-92 his3-11, 15ade2-1 can1-100 GAL SUC mal | Thomas and Rothstein (1989) |
| PM941 | W303-1A | pde1Δ | MATa leu2-3, 112 ura3-1 trp1-92 his3-11, 15 ade2-1 can1-100 GAL SUC mal pde1∷URA3 | This work |
| PM942 | W303-1A | pde2Δ | MATa leu2-3, 112 ura3-1 trp 1-92 his3-11, 15 ade2-1 can 1-100 GAL SUC mal pde2∷URA3 | This work |
| PM943 | W303-1A | pde1Δ pde2Δ | MATa leu2-3, 112 ura3-1 trp1-92 his3-11, 15 ade2-1 can1-100 GAL SUC mal pde1∷TRP1 pde2∷URA3 | This work |
| PM850 | W303-1A | wild type + YEplac195 | MATa leu2-3, 112 ura3-1 trp1-92 his3-11, 15 ade2-1 can1-100 GAL SUC mal + YEplac195 (URA3) | This work |
| PM851 | W303-1A | wild type + YEpPDE1 | MATa leu2-3, 112 ura3-1 trp1-92 his3-11, 15 ade2-1 can1-100 GAL SUC mal + YEpPDE1 (URA3) | This work |
| PM852 | W303-1A | wild type + YEpPDE2 | MATa leu2-3, 112 ura3-1 trp1-92 his3-11, 15 ade2-1 can1-100 GAL SUC mal + YEpPDE2 (URA3) | This work |
| PM581 | W303-1A | pde1ala252 | MATa leu2-3, 112 ura3-1 trp1-92 his3-11, 15 ade2-1 can1-100 GAL SUC mal pde1ala252 | This work |
| PM582 | W303-1A | pde1ala252 pde2Δ | MATa leu2-3, 112 ura3-1 trp1-92 his3-11, 15 ade2-1 can1-100 GAL SUC mal pde1ala252 pde2∷URA3 | This work |
| PM541 | W303-1A | pde1Δ pde2Δ + YCpPDE1 | MATa leu2-3, 112 ura3-1 trp1-92 his3-11, 15 ade2-1 can1-100 GAL SUC mal pde1∷TRP1 pde2∷URA3 + YCpL-PDE1 | This work |
| PM542 | W303-1A | pde1Δ pde2Δ + YCppde1ala252 | MATa leu2-3, 112 ura3-1 trp1-92 his3-11, 15 ade2-1 can1-100 GAL SUC mal pde1∷TRP1 pde2∷URA3 + YCpL-pde1ala252 | This work |
| TK161-R2V | SP1 | RAS2val19 | MATa leu2 his3 trp1 ade8 can1 ura3 RAS2val19 | Broek et al. (1985) |
| DC124 | SP1 | wild type | Matα his4 leu2 ura3 trp1 ade8 can1 | M. Wigler (Cold Spring Harbor) |
| PM944 | SP1 | RAS2val19pde1Δ | MATa leu2 his3 trp1 ade8 can1 ura3 RAS2val19 pde1∷URA3 | This work |
| PM945 | SP1 | RAS2val19pde2Δ | MATa leu2 his3 trp1 ade8 can1 ura3 RAS2val19 pde2∷URA3 | This work |
| PM946 | SP1 | RAS2val19pde1Δ pde2Δ | MATa leu2 his3 trp1 ade8 can1 ura3 RAS2val19 pde1∷TRP1 pde2∷URA3 | This work |
| PM584 | SP1 | RAS2val19pde1ala252 | MATa leu2 his3 trp1 ade8 can1 ura3 RAS2val19 pde1ala252 | This work |
| PM586 | SP1 | RAS2val19pde1ala252pde2Δ | MATa leu2 his3 trp1 ade8 can1 ura3 RAS2val19 pde1ala252pde2∷URA3 | This work |
| J105 | SP1 | pde1Δ | MATa leu2 his3 ura3 trp1 ade8 can1 pde1∷LEU2 | Nikawa et al. (1987b) |
| J104 | SP1 | pde2Δ | MATa leu2 his3 ura3 trp1 ade8 can1 pde2∷HIS3 | Sass et al. (1986) |
| J106 | SP1 | pde1Δ pde2Δ | MATa leu2 his3 ura3 trp1 ade8 can1 pde1∷URA3 pde2∷HIS3 | Nikawa et al. (1987b) |
| PM975 | SP1 | tpk1w1tpk2 tpk3 bcy1 + | MATa his3 leu2 ura3 trp1 ade8 tpk1w1 tpk2∷HIS3 tpk3∷TRP1 | Nikawa et al. (1987a) |
| YCplac33 | bcy1∷LEU2 (RS13-58A-1) + YCplac33(URA3) | This work | ||
| PM976 | SP1 | tpk1w1tpk2 tpk3 bcy1 + YCpPDE1 | MATa his3 leu2 ura3 trp1 ade8 tpk1w1 tpk2∷HIS3 tpk3∷TRP1 bcy1∷LEU2 (RS13-58A-1) + YCpU-PDE1 | This work |
| PM977 | SP1 | tpk1w1tpk2 tpk3 bcy1 + YCppde1ala252 | MATa his3 leu2 ura3 trp1 ade8 tpk1w1 tpk2∷HIS3 tpk3∷TRP1 bcy1∷LEU2 (RS13-58A-1) + YCpU-pde1ala252 | This work |
| PM978 | SP1 | tpk1w1tpk2 tpk3 bcy1 + YCpPDE2 | MATa his3 leu2 ura3 trp1 ade8 tpk1w1 tpk2∷HIS3 tpk3∷TRP1 bcy1∷LEU2 (RS13-58A-1) + YCpPDE2 (URA3) | This work |
| PM979 | SP1 | tpk1w1tpk2 tpk3 bcy1 + YCppde1asp252 | MATa his3 leu2 ura3 trp1 ade8 tpk1w1 tpk2∷HIS3 tpk3∷TRP1 bcy1∷LEU2 (RS13-58A-1) + YCpU-pde1asp252 | This work |
| PM545 | W303-1A | PDE1-HA | MATa leu2-3, 112 ura3-1 trp1-92 his3-11, 15 ade2-1 can1-100 GAL SUC mal-PDE1-HA | This work |
| PM546 | W303-1A | PDE1S252A-HA | MATa leu2-3, 112 ura3-1 trp1-92 his3-11, 15 ade2-1 can1-100 GAL SUC mal pde1ala252-HA | This work |