Table 2.
Kinetic parameters for current simulations
| 5-HT alone | 5-HT + 5-HI | |
|---|---|---|
| 5-HT* | 30 μM | 30 μM |
| k1 | 1 × 107 M−1s−1 | 1 × 107 M−1s−1 |
| k2 | 10 s−1 | 10 s−1 |
| k3 | 0.01 s−1 | 0.01 s−1 |
| β | 400 s−1 | 400 s−1 |
| α | 1 s−1 | 1 s−1 |
| kd+ | 0.5 s−1 | 0.04 s−1 |
| kd− | 0.01 s−1 | 0.01 s−1 |
| kDR | 0.7 s−1 | 0.7 s−1 |
| L0 | 3 × 106 | 3 × 106 |
5-HT concentration and rate constants used to generate simulated 5-HT3A receptor-mediated currents (Figure 10). Note that 5-HT concentration applies only to current simulation of response to continuous agonist application (Figure 10B), while current in simulated “deactivation” protocol run in agonist-free condition (Figure 10C).