Table 3.
Nature and selective value of mutations arising during aging
|
rpoB mutation |
No. of occurrences |
Competitive index (CI) |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| No. | Substitution | Day 1 | Day 7, nonjackpot | Day 7, jackpot | CI | Dev* | N† | P value‡ | |
| 1 | D516G | 14 | 8 | 14 | 2.7 | 2.3 | 11 | 0.0002 | |
| 2 | S531F | 10 | 3 | 0 | 1.0 | 0.4 | 12 | 0.1362 | |
| 3 | H526Y | 5 | 3 | 8 | 1.1 | 7.6 | 14 | 0.6312 | |
| 4 | S512P | 3 | 5 | 3 | 1.1 | 0.4 | 16 | 0.8103 | |
| 5 | R529H | 3 | 4 | 0 | 5.2 | 3.6 | 8 | <0.0001 | |
| 6 | P564L | 3 | 4 | 4 | 27.8 | 93.2 | 11 | <0.0001 | |
| 7 | S522F | 2 | 3 | 0 | 0.2 | 0.7 | 12 | 0.0014 | |
| 8 | I572F | 2 | 1 | 0 | 1.7 | 0.7 | 5 | 0.1188 | |
| 9 | K504N | 1 | 1 | 0 | 3.0 | 1.0 | 11 | <0.0001 | |
| 10 | L511P | 1 | 1 | 0 | 3.1 | 1.5 | 11 | <0.0001 | |
| 11 | S512F | 1 | 4 | 2 | 0.9 | 0.3 | 17 | 0.2891 | |
| 12 | R529C | 1 | 6 | 1 | 17.2 | 7.2 | 16 | <0.0001 | |
| 13 | S512Y | 0 | 2 | 0 | 0.8 | 0.6 | 18 | 0.3628 | |
| 14 | Q513L | 0 | 2 | 0 | 5.7 | 3.7 | 13 | 0.0061 | |
| 15 | Q513H | 0 | 1 | 0 | 12.9 | 5.9 | 15 | 0.0001 | |
| 16 | D516Y | 0 | 2 | 0 | 2.2 | 0.9 | 23 | <0.0001 | |
| 17 | S522Y | 0 | 0 | 1 | 4.6 | 8.0 | 13 | 0.0105 | |
| 18 | H526L | 0 | 2 | 0 | 1.4 | 1.0 | 12 | 0.2041 | |
| 19 | H526P | 0 | 1 | 0 | 1.3 | 2.3 | 25 | 0.2225 | |
| 20 | R529S | 0 | 1 | 0 | 40.6 | 45 | 12 | <0.0001 | |
| 21 | R529L | 0 | 1 | 1 | 8.1 | 9.4 | 18 | <0.0001 | |
*Dev is the absolute average deviation from the median (see Materials and Methods). Note: the CI was positive for all competitions.
†N is the number of independent colony competitions for each mutant.
‡Two-tailed P values (CI of mutant significantly different from CI of wild-type) measured by Mann–Whitney nonparametric test.