Table 2. Polymorphisms observed in seven protein-encoding genes among 50 E. cloacae complex isolates.
Clade 1 (n = 30) | Length (bp) | No of variable sites | no. of haplotypes | π | Tajima's D | Fu's Fs | |||
θ (Total) | Total | synonymous changes | non-synonymous changes | ||||||
included for analysis | |||||||||
fusA | 633 | 11 | 9 | 0.0044 | 0.0039 | 0.0153 | 0.0006 | −0.3246 | −1.339 |
gyrB | 417 | 43 | 18 | 0.0297 | 0.0284 | 0.0910 | 0.0075 | −0.1594 | −1.437 |
leuS | 642 | 54 | 19 | 0.0232 | 0.0201 | 0.0817 | 0.0008 | −0.5045 | −1.761 |
pyrG | 306 | 8 | 8 | 0.0066 | 0.0056 | 0.0227 | 0.0000 | −0.4510 | −1.817 |
rpoB | 501 | 21 | 14 | 0.0106 | 0.0089 | 0.0351 | 0.0000 | −0.5739 | −3.083 |
hsp60 | 273 | 26 | 10 | 0.0259 | 0.0234 | 0.0842 | 0.0022 | −0.0351 | 1.387 |
Mean | 2772 | 163 | 25 | 0.0160 | 0.0143a | 0.0538 | 0.0017 | −0.4061 | −1.391 |
excluded from further analyses | |||||||||
rplB | 333 | 4 | 6 | 0.0038 | 0.0012 | 0.0043 | 0.0000 | −1.87843b | −4.834c |
Clade 2 (n = 20) | |||||||||
included for analysis | |||||||||
fusA | 633 | 21 | 11 | 0.0098 | 0.0087 | 0.0319 | 0.0018 | −0.4267 | −1.364 |
gyrB | 417 | 90 | 19 | 0.0737 | 0.0671 | 0.2241 | 0.0141 | −0.3705 | −3.526 |
leuS | 642 | 157 | 20 | 0.0053 | 0.0947 | 0.3399 | 0.0175 | 0.1523 | −2.776 |
pyrG | 306 | 32 | 15 | 0.0350 | 0.0418 | 0.1476 | 0.0074 | 0.7711 | −1.783 |
rpoB | 501 | 32 | 18 | 0.0191 | 0.0173 | 0.0686 | 0.0000 | −0.0384 | −8.191c |
hsp60 | 273 | 44 | 17 | 0.0578 | 0.0507 | 0.1719 | 0.0079 | −0.5243 | −3.707 |
Mean | 2772 | 376 | 20 | 0.0475 | 0.0467a | 0.1663 | 0.0082 | −0.0700 | −1.116 |
excluded from further analyses | |||||||||
rplB | 333 | 9 | 7 | 0.0076 | 0.0097 | 0.0357 | 0.0000 | 0.9347 | 0.229 |
Difference indicates that clade 2 is older than clade 1.
P<0.05.
P<0.01.