Table 2. Polymorphisms observed in seven protein-encoding genes among 50 E. cloacae complex isolates.

Clade 1 (n = 30)	Length (bp)	No of variable sites	no. of haplotypes	π				Tajima's D	Fu's Fs
				θ (Total)	Total	synonymous changes	non-synonymous changes
included for analysis
fusA	633	11	9	0.0044	0.0039	0.0153	0.0006	−0.3246	−1.339
gyrB	417	43	18	0.0297	0.0284	0.0910	0.0075	−0.1594	−1.437
leuS	642	54	19	0.0232	0.0201	0.0817	0.0008	−0.5045	−1.761
pyrG	306	8	8	0.0066	0.0056	0.0227	0.0000	−0.4510	−1.817
rpoB	501	21	14	0.0106	0.0089	0.0351	0.0000	−0.5739	−3.083
hsp60	273	26	10	0.0259	0.0234	0.0842	0.0022	−0.0351	1.387
Mean	2772	163	25	0.0160	0.0143a	0.0538	0.0017	−0.4061	−1.391
excluded from further analyses
rplB	333	4	6	0.0038	0.0012	0.0043	0.0000	−1.87843b	−4.834c
Clade 2 (n = 20)
included for analysis
fusA	633	21	11	0.0098	0.0087	0.0319	0.0018	−0.4267	−1.364
gyrB	417	90	19	0.0737	0.0671	0.2241	0.0141	−0.3705	−3.526
leuS	642	157	20	0.0053	0.0947	0.3399	0.0175	0.1523	−2.776
pyrG	306	32	15	0.0350	0.0418	0.1476	0.0074	0.7711	−1.783
rpoB	501	32	18	0.0191	0.0173	0.0686	0.0000	−0.0384	−8.191c
hsp60	273	44	17	0.0578	0.0507	0.1719	0.0079	−0.5243	−3.707
Mean	2772	376	20	0.0475	0.0467a	0.1663	0.0082	−0.0700	−1.116
excluded from further analyses
rplB	333	9	7	0.0076	0.0097	0.0357	0.0000	0.9347	0.229

^aDifference indicates that clade 2 is older than clade 1.

^bP<0.05.

^cP<0.01.