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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Cell Microbiol. 2007 Sep 10;10(2):332–343. doi: 10.1111/j.1462-5822.2007.01044.x

Figure 1. Protection from LT-induced macrophage death with caspase inhibitors.

Figure 1

(A) RAW264.7 cells and BALB/cJ BMDM were treated with caspase inhibitors for 1 h prior to LT addition (1 μg/ml), and cell viability was assessed by staining with MTT at 3 h. In a similar experiment, ZVAD-FMK was added to RAW264.7 cells 1 h prior to LT addition (1 μg/ml), at the same time as LT addition, or 40 min after LT addition. Data shown are from a single experiment representative of the protection curves from five separate assays. (B) LPS-primed RAW264.7 cells and LPSprimed BALB/cJ BMDM were pretreated with the indicated caspase inhibitors (all at 100 μM, 1 h) prior to LT treatment (1 μg/ml) for 70 min (RAW) or 85 min (BMDM). Cell lysates were prepared and analyzed for the presence of the p10 fragment of active caspase-1 or mature IL-1β by Western blotting.