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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Cell Microbiol. 2007 Sep 10;10(2):332–343. doi: 10.1111/j.1462-5822.2007.01044.x

Figure 3. Inflammasome formation is prevented in the presence of KCl, sucrose, quinidine and TEA.

Figure 3

(A) BALB/cJ BMDM were treated with LT (1 μg/ml) for the indicated times (min) in DMEM alone or DMEM containing 130 mM KCl or 300 mM sucrose. In the indicated control lanes, cells were primed with LPS (1 μg/ml; 2 h) and treated with 20 μM nigericin for 25 min. Caspase-1 activation and IL-18 maturation were monitored in cell lysates by Western blotting. (B) RAW264.7 cells were treated with 1 μg/ml LT in the presence or absence of 130 mM KCl or 300 mM sucrose for 2.5 h. Cells were then stained with MTT, and pictures of stained cells were taken using a light microscope. (C) BALB/cJ BMDM were pretreated with quinidine or TEA at the indicated concentrations for 25 min prior to LT treatment (1 μg/ml) for 85 min. Active caspase-1 and mature IL-18 were then detected in cell lysates by Western blotting. In all gels, NT refers to no-treatment control cells.