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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Cell Microbiol. 2007 Sep 10;10(2):332–343. doi: 10.1111/j.1462-5822.2007.01044.x

Figure 4. LT-induced caspase-1 activation requires active proteasomes.

Figure 4

(A) BALB/cJ BMDM were pretreated with lactacystin (15 μM) for 30 min prior LT treatment (1 μg/ml) for the indicated times (min). Active caspase-1 was detected in cell lysates by Western blot. (B) BALB/cJ BMDM were primed with LPS (1 μg/ml) for 1.5 h prior to lactacystin (15 μM) addition 30 min prior to addition of 20 μM nigericin for 25 min. Mature IL-1β was detected in cell lysates by Western blot.