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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Cell Microbiol. 2007 Sep 10;10(2):332–343. doi: 10.1111/j.1462-5822.2007.01044.x

Figure 5. Caspase-1 activation alone does not sensitize resistant macrophages to LT.

Figure 5

(A) C57BL/6J BMDM were primed with LPS for 2 h, followed by medium removal, and addition of nigericin (5 μM) for 25 min. Caspase-1 and IL-1β were monitored in cell lysates by Western blotting. (B) DBA/2J and C57BL/6J BMDM were treated with LPS and 5 μM nigericin as in (A). LT was then added, and cell viability was determined 2.5 h later by MTT staining.