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. 1999 Jan;10(1):119–134. doi: 10.1091/mbc.10.1.119

Figure 10.

Figure 10

GS32 exists in a 20-S complex that contains NSF and α-SNAP. The 20-S fractions were immunoprecipitated with antibodies against GS32 (lanes 1–4) or control rabbit IgG (lanes 5–8). The immunoprecipitates were eluted with assembly buffer (lanes 1, 3, 5, and 7) or disassembly buffer (lanes 2, 4, 6, and 8). The beads (lanes 1, 2, 5, and 6) and eluates (lanes 3, 4, 7, and 8), together with 100 ng of NSF and α-SNAP (lane 9), were analyzed by immunoblot to detect NSF and α-SNAP. NSF and α-SNAP were associated with the beads (lane 1) but not the eluate (lane 3) in assembly buffer. In disassembly buffer, the majority of NSF and α-SNAP was released into the eluate (lane 4), with a small portion remaining associated with the beads (lane 2).