Figure 13.

Antibodies against GS32 but not GS28 coimmunoprecipitate syntaxin 6 from Golgi extracts. Golgi extracts were immunoprecipitated with antibodies against GS32, GS28 or with control rabbit IgG. The immunoprecipitates (lanes 1, 2, and 3) and postimmunoprecipitate supernatants (lanes 4, 5, and 6) were resolved by SDS-PAGE and processed for immunoblot to detect GS32 (A), syntaxin 5 (B), and syntaxin 6 (C). As shown, syntaxin 6 but not syntaxin 5 was selectively coimmunoprecipitated by GS32-specific antibodies. Lanes 1–3 show the beads, whereas lanes 4–6 show 20% of the postimmunoprecipitated supernatants.