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. Author manuscript; available in PMC: 2008 Aug 13.
Published in final edited form as: FASEB J. 2008 Jan 7;22(5):1450–1457. doi: 10.1096/fj.07-9309com

Figure 4.

Figure 4

Dynamic subcellular localization of α-actinin-4 and eNOS in endothelial cells. A) Immunofluorescence localization of eNOS and α-actinin-4 on HSVECs. The cells were double stained for eNOS (green) and α-actinin-4 (red), and analyzed with a confocal microscope. Merged image shows a colocalization of eNOS and α-actinin-4. Right panel: magnification of colocalized area. B) Immunofluorescence localization of eNOS and α-actinin-1. The cells were double stained for eNOS (green) and α-actinin-1 (red). Merged image does not show colocalization of eNOS and α-actinin-1. C) Dynamic movement of α-actinin-4 induced by Ca2+ ionophore. Small amount of α-actinin-4 was localized at cell membrane before stimulation. Stimulation with A23187 (10 μM) decreased membrane localization of α-actinin-4 within 1 min. Scale bars = 25 μm.